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When cells were X-irradiated, the medium was replaced with a medium containing 25 mM HEPES to maintain the pH in the absence of CO 2 , and after X-irradiation, cells were put back into normal culture condition, i.e., in the presence of 5% CO 2 , and the medium was, thus, replaced by a HEPES-free medium

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This was a velvet, off-the-shoulder minidress that she accessorised with a sapphire choker in an ensemble that became one of fashions most memorable moments instantly